One of three books I continuously referred to throughout the year as I worked on my thesis. It's pretty hard to oversell just how important Category Theory has become in mathematics. For something that was essentially formalised in the 1950s — a small-little baby when compared to the geriatric status of most other theories in mathematics — it's incredible how quickly it has become ubiquitous. Seriously. There's not a single modern mathematics textbook that doesn't give you a quick primer on functors, natural transformations, adjoints, duals, etc. It is just essential to get anywhere nowadays in maths. The problem with learning about Category Theory in this ad-hoc fashion, is that the definitions feel utterly austere. If you're looking for any more information, intuition, or justification, then bad luck — go look elsewhere.
So, what the hell do-you-do if you actually need to learn the nuts-and-bolts of the damn thing? You probably go to one of the two guys that literally created it: Mac Lane. Who else could do it better than the person who came up with the all the definitions, axioms and view points? This is no doubt one of the reasons why everyone who is even slightly interested in maths, knows about this book: it's a treat to have, as your tour-guide, the person who laid down all the bricks in the palace you now stand in. The problem, however, is that Mac Lane created fucking Category Theory. He naturally sees so much further than everyone-else that he has forgotten the problems of the myopic-riddled masses. He'll be describing to you the beautiful mountain range 3000kms away that perfectly and succinctly explains his point, whilst you're still trying to poke your head out from under the dense foliage.
If you're in dire need of a reference to all things Category Theory, then look no further. If you need absolutely anything else — barring some trifle that you wish resolved by the most authoritative voice on the matter — then this really isn't worth your time.