MicroRNA Expression Detection Methods

by Zhiguo Wang, Baofeng Yang

General Remarks.- Detection, Profiling, and Quantification of miRNA Expression.- miRNA Microarray Methods.- Microarray and Its Variants for miRNA Profiling.- Northern Blotting Methods.- Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs.- In Situ Hybridization Methods.- In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs.- Real-Time RT-PCR Methods.- End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification.- miR-Q RT-PCR for miRNA Quantification.- Poly(A)-Tailed Universal Reverse Transcription.- Multiplexing RT-PCR for High-Throughput miRNA Profiling.- miRNA Amplification Profiling (mRAP).- Cloning Methods.- miRNA Serial Analysis of Gene Expression (miRAGE or SAGE).- Nanoparticle Methods.- Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis.- Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling.- Conducting Polymer Nanowires Technique for High-Sensitivity miRNA Expression Analysis.- Gold Nanoparticle Probe Method for miRNA Quantification.- Other Methods.- Splinted Ligation Method.- Padlock-Probes and Rolling-Circle Amplification.- Invader Assay.- Single Molecule Method.- Enzymatic Method.- Surface-Enhanced Raman Spectroscopy Method.- RAKE Assay.- Bead-Based Flow Cytometric miRNA Expression Profiling.- Bioluminescence miRNA Detection Method.- Molecular Beacon Method.- Ribozyme Method.- Electrocatalytic Moiety Labeling Technique for High-Sensitivity miRNA Expression Analysis.- Circulating miRNA Detection Methods.- Serum and Plasma miRNA Detection.- miRNA Detection from Peripheral Blood Microvesicles.- Detection of Placental miRNAs in Maternal Plasma.- Single-cell miRNA Detection Methods.- Quantitative LNA-ELF-FISH Method for miRNA Detection in Single Mammalian Cell.- Single Cell Stem-Looped Real-Time PCR.- miRNA Function-Reporter Expression Assay.- Whole Mount In Situ Analysis.- Whole Mount In Situ Hybridization (WM-ISH) for miRNA Expression Profiling During Vertebrate Development.

408 pages, Paperback

First published December 23, 2009