Methods in Enzymology, Volume 152: Guide to Molecular Cloning Techniques

by Shelby L. Berger (Editor), Alan R. Kimmel (Editor)

This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.
Key Features
* Comprehensive protocols compiled specifically for this volume
* Among the topics covered:
* Genomic cloning
* Preparation and characterization of mRNA
cDNA cloning
* Screening libraries
* Identification and characterization of clones
* Methods written by 87 experts
* Editors' notes, overviews, and extensive cross-referencing
* Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility
* A problem-solving quantitative approach
* Emphasis on strategies for choosing alternative methods

Hardcover

First published September 28, 1987