Biochemistry Laboratory Practical Manual for Phase-1 MBBS Students

by Pankaja Naik

Section Quantitative Experiments (Practical/DOAP)
Estimation of serum total cholesterol
Estimation of serum HDL cholesterol
Estimation of triglycerides
Estimation of calcium
Estimation of phosphorous
Estimation of serum bilirubin
Estimation of ALT
Estimation of AST
Estimation of alkaline phosphatase
Estimation of blood glucose
Estimation of blood urea
Estimation of serum creatinine and creatinine clearance
Estimation of serum total protein, albumin and A/G ratio

Section Qualitative Experiments (DOAP/SGD)
Physical and chemical components of normal and abnormal urine
Analysis of normal urine
Identify abnormal constituents in urine, interpret the findings and correlate these with pathological states

Section Quality Control (Demo)

Section Interpretation of Laboratory Reports (SGD)
Basis and rational of biochemical tests done in various
Experiment No. Competency No. Competency Page No. Date Sign

Section Interpretation of Laboratory Reports Based on Special Technique (SGD)
Protein electrophoresis and PAGE
Paper chromatography and TLC
Screening of urine for inborn errors and the use of paper chromatography
ABG analyzer

Section Spotters (SGD)
Common laboratory Glassware and equipment
Sample collection
Good and safe laboratory practice
Biomedical waste management in laboratory pH meter and preparation of buffers
Principles of colorimetry
Principles of spectrophotometry
Electrolyte analysis by ISE
Enzyme-linked immunosorbent assay Immunodiffusion DNA Isolation Autoanalyzer
Composition of cerebrospinal fluid

Practical Exam Ist, IInd, IIIrd Internal Assessment and University
Practical Question Paper Ist, IInd, IIIrd Internal Assessment and University

262 pages, Paperback

Published December 1, 2022