Professionals in the liquid chromatography business and those providing bioanalytical contract services to the pharmaceutical industry will learn to solve liquid chromatography problems like leaks and pressure by using this troubleshooting guide to liquid chromatography for method development, compiled from a variety or resource materials supplied by LCGC North America magazine and Chromatographyonline.com.
Liquid chromatography is a separation technique in which the mobile phase is a liquid. Liquid chromatography can be conducted either in a column or a plane, the premier global resource for unbiased, peer-reviewed technical information on the field of chromatography and the separation sciences.
Nearly every chromatographer needs to do some kind of method development at one time or another. Whether the job is running a routine liquid chromatography method that needs an occasional 'tweak,' there is the need to develop a one-use method to support chemical synthesis, or a robust method to monitor a production process.
John Dolan's titling of this series 'The Perfect Method' is a little tongue-in-cheek, because in his experience there is no such thing as a 'perfect' 'every method I have seen,' Dolan says, 'can always be made better.' Herein lies the first principle of method 'better is the enemy of good enough.'