RT-PCR Protocols

by Joe O'Connell

In view of the ubiquity of the use of standard RT-PCR, the main objective of this volume is to provide novel, useful applications of RT-PCR. These include some useful adaptions and application that are relevant to the wider research communities who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptions are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imbalances in specific mRNA expression between normal and diseased tissues. RT-PCR has afforded many opportunities in diagnostics, allowing sensitive detection of RNA viruses such as HIV and HCV. RT-PCR facilities many diverse techniques in research, including in situ localization of mRNA, antibody engineering, and cDNA cloning. In particular, this volume highlights how RT-PCR, microarray technology, HPLC and time-resolved fluorimetry. This volume will stimulate the reader towards diverse new ways in which this remarkable technique can facilitate the molecular aspects of their biomedical research.

380 pages, Hardcover

First published July 15, 2002