DNA Barcoding: Methods and Protocols

by Rob DeSalle (Editor)

This volume covers the latest advancements and methods used to study DNA barcoding. The chapters in this book are organized into six theory, DNA manipulation, specialized barcoding methods, analysis of DNA barcodes, completing a successful DNA barcode project, and barcoding as citizen science. Part One looks at several theoretical issues in DNA barcoding concerned with DNA barcodes that can and should be used. Part Two discusses protocols for processing samples into DNA barcodes, including DNA isolation, sequencing and managing samples, and the resulting DNA barcodes. Part Three explores the next generation of DNA barcoding such as FRET-based detection, dual nanopore detection, skimming, and closed-tube methods. Part Four addresses programs and websites that handle DNA barcoding data, and includes character-based approaches, barcode gap methods, integrated approaches, and Barcode of Life Database. Part Five offers protocols for producing a successfulDNA barcoding project including plant and animal examples and protocols for generating a DNA Barcode Reference Library. Lastly, Part Six talks about several novel protocols for setting up an educational program using DNA barcodes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, DNA  Methods and Protocols encompasses a wide range of techniques and will be a useful reference tool for both scientist and student.




 

595 pages, Hardcover

Published April 30, 2024

About the author

Rob DeSalle is curator of entomology in the Sackler Institute for Comparative Genomics at the American Museum of Natural History, New York City. He is author or coauthor of dozens of books, several based upon exhibitions at the AMNH, including The Brain: Big Bangs, Behaviors, and Beliefs and A Natural History of Wine, coauthored with Ian Tattersall and published by Yale University Press. He lives in New York City.