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Phytochemical screening of Datura using GC-MS in Shivalik and mid- Himalayan regions of Himachal Pradesh
This study has attempted phytochemical screening of Datura in Himachal Pradesh based on altitude differences. We have also attempted to provide proof of differences in the concentrations of phytochemicals within the same species as is stated in previous literature . In this study, Datura stramonium was taken into consideration as an important toxic plant encountered in poisoning and abuse cases in Himachal Pradesh. Datura specimens were collected from selective regions of Himachal and were grouped according to the altitude.
They were carefully dried and subjected to microscopic examination for studying various botanical features, alkaloid identification, and phytochemical screening for the purpose of identification. The phytochemical screening was done by using GC-MS. The alkaloid content of the samples exhibited variability. The samples from lower altitude regions had a lower content of Scopolamine in comparison to that of the higher altitude. The present study attempted to analyze various alkaloids present in Datura stramonium and also distinguish the alkaloid concentrations with correlation to the altitude difference. The GC-MS analysis identified atropine and scopolamine, which are very fatal and can eventually cause death. This data can help in better forensic profiling of Datura. This may prove helpful in studying the origin of forensic evidence obtained at the scene of the crime.
They were carefully dried and subjected to microscopic examination for studying various botanical features, alkaloid identification, and phytochemical screening for the purpose of identification. The phytochemical screening was done by using GC-MS. The alkaloid content of the samples exhibited variability. The samples from lower altitude regions had a lower content of Scopolamine in comparison to that of the higher altitude. The present study attempted to analyze various alkaloids present in Datura stramonium and also distinguish the alkaloid concentrations with correlation to the altitude difference. The GC-MS analysis identified atropine and scopolamine, which are very fatal and can eventually cause death. This data can help in better forensic profiling of Datura. This may prove helpful in studying the origin of forensic evidence obtained at the scene of the crime.
25 pages, Kindle Edition
Published October 18, 2024
About the author
Priya Sharma
146 books243 followersPriya Sharma’s fiction has appeared venues such as Interzone, Black Static, Nightmare, The Dark and Tor. “Fabulous Beasts” was a Shirley Jackson Award finalist and won a British Fantasy Award for Short Fiction. Priya is a Shirley Jackson Award and British Fantasy Award winner, and Locus Award finalist, for “All the Fabulous Beasts”, a collection of her some of her work, available from Undertow Publications.
“Ormeshadow”, her first novella (available from Tor), won a Shirley Jackson Award and a British Fantasy Award. It was a 2022 Grand Prix de l’Imaginaire finalist.
"Pomegranates", her second novella (from Absinthe, an imprint of PS Publishing) is a Shirley Jackson Award, British Fantasy Award finalist and won a World Fantasy Award.
Her stories have been translated into Spanish, French, Italian, Czech, and Polish.
“Ormeshadow”, her first novella (available from Tor), won a Shirley Jackson Award and a British Fantasy Award. It was a 2022 Grand Prix de l’Imaginaire finalist.
"Pomegranates", her second novella (from Absinthe, an imprint of PS Publishing) is a Shirley Jackson Award, British Fantasy Award finalist and won a World Fantasy Award.
Her stories have been translated into Spanish, French, Italian, Czech, and Polish.
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