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Practical NGS Data Analysis: A Step-by-Step Lab Manual
This laboratory manual has been meticulously designed to introduce postgraduate students to the practical skills and analytical approaches in Next-Generation Sequencing (NGS) data analysis. Each module follows a step-by-step protocol to ensure clarity and reproducibility, empowering students to master complex bioinformatics workflows with confidence.
The manual includes hands-on exercises covering raw data preprocessing, reference mapping, variant calling, RNA-Seq, differential gene expression, and metagenomic analysis. Each module also includes assignments and real datasets to encourage problem-solving and critical thinking. Appendices at the end offer cheat sheets, Linux command guides.
I hope this manual becomes a valuable resource for students, educators, and researchers exploring the world of functional genomics and computational biology.
Dr. Anish Kumar
M.Sc, Ph.D., PGDIPR
The manual includes hands-on exercises covering raw data preprocessing, reference mapping, variant calling, RNA-Seq, differential gene expression, and metagenomic analysis. Each module also includes assignments and real datasets to encourage problem-solving and critical thinking. Appendices at the end offer cheat sheets, Linux command guides.
I hope this manual becomes a valuable resource for students, educators, and researchers exploring the world of functional genomics and computational biology.
Dr. Anish Kumar
M.Sc, Ph.D., PGDIPR
83 pages, Kindle Edition
Published August 7, 2025
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