Cooperativity and Regulation in Biochemical Processes

by Arieh Ben-Naim

1. Introducing the Fundamental Concept. 2. The Binding Isotherm (BI). 3. Adsorption on a Single Polymer with Conformation Changes Induced by the Binding Process. 4. Two-site Systems; Direct and Indirect Cooperativity. 5. Three-site Systems; Non-additivity and Long Range Correlations. 6. 7. Large Linear Systems of Binding Sites. 8. Regulatory Enzymes. 9. Solvent Effects on Cooperativity. 10. Appendices. Appendix Pair and triple correlations between events. Appendix Localization of the adsorbent molecules, and its effect on the binding isotherm. Appendix Transition from microstates to macrostates. Appendix First order correction to non-ideality of the ligand's reservoir. Appendix Relative slopes of the equilibrated and 'frozen in' BI's in a multi macrostate system. Appendix Spurious cooperativity in single-site systems. Appendix The relation between the binding isotherm and the titration curves for two-site systems. Appendix Synthetic data. Appendix A comment on nomenclature. Appendix Average binding constants and correlation functions. Appendix Utility function in binding systems.

368 pages, Paperback

First published February 28, 2001