A-Z of Quantitative PCR

by Stephen A. Bustin

Bustin (School of Medicine and Dentistry, University of London, UK) and contributors explain features of quantitative PCR (qPCR) and provide alternative viewpoints and methods in this hands-on manual for novice and expert researchers who want to learn how to set up and carry out qPCR assays from scratch. Early chapters give insight on factors that influence a PCR experiment and principles underlying real-time quantification. The core of the book details the steps involved in conducting a qPCR experiment, from sample collection to interpretation of results. Final chapters provide a protocol for mRNA quantification and guidelines for standardization of qPCR assays. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)

910 pages, Hardcover

First published July 1, 2004